FIB-milling and cellular tomography (cryo-ET)

As part of our cryo-CLEM approach, we utilise focused ion beam (FIB) milling and cryo-electron tomography (cryo-ET) in collaboration with eBiC (Diamond Light Source) to investigate intricate structures of prion assemblies within cells and tissues at sub-nanometre resolution. This cutting-edge approach will provide unprecedented insights into the structural organization of prion assemblies in their native environment, i.e. in specific (peri/sub)cellular compartments. It will thus shed new light on prion infection pathways, ultimately contributing to our understanding of the pathogenicity mechanisms driving prion (and prion-like) diseases and to the development of potential therapeutic interventions.

We are seeking a cryo-ET and sub-tomogram averaging expert to join our team!